REST2009 is a popular bioinformatics tool to analyze relevant geneexpression changes from crossing points (CP) of amplification curvesgathered by a Real-Time PCR (qPCR) experiment. REST was developed byMichael W. Pfaffl (Technical University Munich) and QIAGEN.

However,I found REST 2009 (v2.0.13) to cease working when moving from a 32bitWindows operating system (Windows XP) to a 64bit Windows operatingsystem (Windows 7). The accepted solution until now was, to install aVirtualPC software including a Windows XP image optimized to run underWindows 7 (dubbed Windows XP Mode). Resourcewise it was a disaster, notto mention the hassle of backing up an entire second system. Also, theneed for additional security software in your virtual image made thesystem even slower, remember Microsoft ceased to provide securityupdates for Windows XP from the 8th of April 2014. Occasionally yourwould need need to install other 3rd party software to complete youranalyses, in my case, I needed to install a PDF printer to be able tosave my REST 2009 reports in a standardazied way. On top of all that,you always need to cope with net drive assignments, virtual net drivesthat will provide access to the underlying OS from inside your virtualXP and that behave a lot different in XP than Windows 7, as well assome restrictions regarding the virtual desktop or virtual windows theVirtualPC software provides. For example, you won't be able to move theemulated REST 2009 windows to a second monitor.

Rotor gene q software download. More Apple Software Update 2.6.0.1. 84.2MB - Shareware - Apple Software Update is a software tool by Apple that installs.

Afterall this complaining about how bad this free functionality in Windows 7is, I want to present my workaround that has since then worked verywell for me.

Downloadsneeded

You will need the following.
• REST 2009installer
  REST 2009 needs to be installed OUTSIDE of the virtual machine, i.e. ina regular manner
  alternativeDownload link
• CorFlags.exe
  which may be obtained by installing the following.NETSDK 3.5. subcomponent(youneed to install the .NET Development tools compnent only)

PatchingREST 2009

Theproblem with REST 2009, which was most probably written in .NET is,that the author missed to flag the code as 32bit code. What happens,when executing undefined code under a 64bit system is, that the code isexecuted as 64bit and thus fails. Under a 32bit system, the 32bit codeworks fine.

UsingCorFlags.exe, it ispossible to subsequently edit those header flags, making a 64bitWindows able to correctly identify and interpret the code.

Afterthe above mentioned software components are installed,CorFlags.exeshould reside in
%PROGRAMFILES%Microsoft SDKsWindowsv6.1bin
whereasRest.exeis located at
%PROGRAMFILES(X86)%REST 2009

Usingan elevated command prompt (hit the WINDOWS key, search for cmd.exe,right-click cmd.exe and select 'Run as administrator...'), simply enterthe following command (or paste the code using the right mouse button):
cmd.exe /c '%PROGRAMFILES%MicrosoftSDKsWindowsv6.1binCorFlags.exe' '%PROGRAMFILES(X86)%REST2009Rest.exe' '/32bit+' & pause
If there is no error message appearing in your command prompt,everything worked as expected and you might try running theRest.exedirectly under your 64bitWindows installation.

Thisinstruction is copyright by Christian Wolf (2015) and may not bereproduced without written permission, instead you may freely link tothis page to help others coping with the same problem.Last updated: 27.05.2015

Rotor Gene Q Series Software Download

Qiagen Rotor-Gene Q PCR Cycler 5 Plex, QIAgility Prep System, Rotor-Disc Sealer | Priced $12,995.00 | bostonind.com (13754)

Here is a Qiagen Rotor-Gene Q PCR Cycler 5 Plex w/ QIAgility Prep System & Rotor-Disc Sealer
Predecessor to:
Current model.
This setup is around $65,000-$75,000 new.
Condition:
This Qiagen Rotor-Gene Q PCR Cycler system is in good working order.
Please see the exact video and photos below.
Testing we performed:
The Qiagen Rotor-Gene Q PCR Cycler system passed all tests and was found to be fully functional:
QIAGEN Rotor-Gene Q Real-Time PCR Cycler testing
Tested Communication with Software: PASSED
Tested Incubation: PASSED
Tested Temperature Ramp: PASSED
Tested Method Editor: PASSED
Tested Centrifuging: PASSED
QIAgility Liquid Handler testing
Tested Communication with software: PASSED
Tested Initialization: PASSED
Tested Self Check Test: PASSED
Tested Method Run: PASSED
Tested Positioning: PASSED
Rotor-Disc Heat Sealer testing
Tested Automatic Function: PASSED
Tested Permanent Seal: PASSED
Tested Removable Seal: PASSED
System consists of:
Qiagen Rotor-Gene Q Real-Time PCR Cycler 5 Plex HRM
Qiagen QIAgility Liquid Handling Robot
Qiagen Roto-Disc Heat Sealer
Dell Latitude i5 running Windows 7 Pro running QIAgility software ver4.17 and Rotor-Gene Q software ver2.0
(2) Rotor Stands
36-vile Rotor
72-vile Rotor
100-vile Rotor
Box of 0.1ml strip tubes Approximately 250 Strips of 4 w/ Lids (P/N 1055470)
200ul PCR Tube, Generic Plate
24-position Reagent Block w/ 72 PCR positions
14-position Reagent Block w/ 72 PCR positions
Loading Block, Rotor-Disc 100 Easylift Ejector
Tip Rack Holder for 96-Tip Rack
Adapter, Flip-Cap 32x1.5ml Tapered-Base
Adapter, 96x0.2ml PCR Tubes
Master Mix Block, 5x5ml Tubes
Loading Block, Rotor-Disc 100 Easylift
Reagent Block, 16x0.2ml/2ml Flat/1.5ml Tapered Tubes
Communication and power cables.
Configuration and Setup:
Rotor-Gene Q Centrifuge Real-Time PCR Cycler
Samples per Run: tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); rotor-disc 72; rotor-disc 100; up to 100 samples per run using a rotor-disc 100
Temperature Range: 35 to 99C
Temperature Accuracy: +/-0.5C
Ramp Speed: >15C/second heating; >20C/second cooling
Dimensions (WxDxH): 370 x 420 x 275mm (14.6 x 16.5 x 10.8in.)
QIAgility Liquid Handling Robot
96 Plate Run Time: from 6 mins (Master mix preparation) to 41 mins (PCR setup)
Temperature Range: 15–30 C (59–86F)
Precision: CV <1%, 5–200 ul (dry-well transfer) CV <5%, 2–5 ul (wet-well transfer) CV <10%, 1 ul
Tip Volume: 1–200 ul
Dimensions (HxWxD): 45 x 55 x 63 cm (17.7 x 21.6 x 24.8 in)
Roto-Disc Heat Sealer
This heat sealer seals complete rings of tubes in the 72 and 100 tube size.
Video Description:
Video #1
This video shows a general overview of the complete Qiagen Rotor-Gene Q PCR Cycler system.
We also view the manufactures tags and software version. Finally we show initialization of the QIAgility Liquid Handling Robot.
Video #2
In this video we start off with an overview of the Qiagen Rotor-Gene Q PCR Cycler.
Then we select the installed rotor, check the locking ring, then start a simple heating run.
Finally we watch the run progress and view the software information.
Video #3
In this video we show an overview of the Rotor-Disc Heat Sealer as well as simulated function.
Documentation:
Datasheet: N/A
Rotor-Gene Q Brochure: https://drive.google.com/file/d/1bMAb4auIVe0EcgDxNA8PfZN8kwCrIK74/view?usp=sharing
Rotor-Gene Q Manual: https://drive.google.com/file/d/1TBMMM26lTR4A2NvFE2uUqwr8RjMzUkji/view?usp=sharing
QIAgility Brochure: https://drive.google.com/file/d/1WhcQ_fgdYA5GI5PgtVp1XHeMuD3mov9H/view?usp=sharing
QIAgility Manual: https://drive.google.com/file/d/1Ri_Hdl60CWPFHYsL2uRCuayntZ_fJOp7/view?usp=sharing
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
Please do not hesitate to contact us if you have any questions.
Video #1

Rotor Gene Q Software For Sale

Video #2

Video #3